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用异基因外周血造血干细胞移植(allo-PBSCT)的方法治疗白血病,正被用以取代骨髓移植。但在施行移植后如何尽早判断移植是否成功,对决定进一步治疗方案至关重要,人类DNA具有高度的遗传多态性,利用特异已知序列的DNA片段与目的DNA杂交所得到的DNA指纹图谱具有高度的个体特异性;而多聚酶链式反应(PCR)技术则是利用靶DNA序列侧翼上所结合的两个寡核苷酸引物经体外酶促合成特异DNA片段,笔者利用较为优秀的D_1S_(80)、D_1S_(111)、apoB及HUMTH01 4个位点的扩增片段长度多态性检测和最新研制的JL-02探针杂交所得的DNA指纹图技术对1例行allo-PBSCT患者的血液在移植后13天进行DNA分型检测,得到了确定其移植是否成功的明确结论,现报道如下。
Treatment of leukemia with allogeneic hematopoietic stem cell transplantation (allo-PBSCT) is being used to replace bone marrow transplantation. However, how to determine the success of the transplant as soon as possible after the transplantation is performed is crucial to the decision for further treatment. Human DNA has a high degree of genetic polymorphism. DNA fingerprints obtained by hybridization of a DNA fragment with a specific known sequence to the target DNA have Highly individual specificity, while polymerase chain reaction (PCR) technology utilizes two oligonucleotide primers flanked by target DNA sequences to enzymatically synthesize specific DNA fragments in vitro. The author used the superior D_1S_(80). ), D_1S_(111), apoB, and HUMTH01 Amplified Fragment Length Polymorphism Detection and DNA Fingerprinting Techniques Obtained from the Newly Developed JL-02 Probe Hybrid Aligned the Blood of a Patient with Allo-PBSCT DNA typing was performed 13 days after transplantation, and clear conclusions were made to determine whether the transplantation was successful. The report is as follows.