论文部分内容阅读
目的 :建立动物模型观察实验性外淋巴瘘 (EPLF)对内耳形态学的影响 ,为临床治疗外淋巴瘘提供实验性依据。方法 :2 4只豚鼠随机分成 6组 ,每组 4只 ,右耳为对照耳。用微型注射器刺破左耳圆窗膜 (RWM ) ,并抽出 2～ 4 μl外淋巴液造成EPLF ,分别于造瘘后 1、2、4、7、1 4和 2 8天处死 ,光镜和电镜下观察内耳形态变化。结果 :穿孔的RWM大部分 ( 83 % )易于愈合。RWM破裂后 2～ 4天穿孔处组织连接 ,7～ 1 4天基本愈合。扫描电镜观察造瘘后 4天有毛细胞损伤 ,瘘口未愈合耳的耳蜗底回毛细胞明显减少。结论 :EPLF转归有二 ,单一RWM穿孔趋向自然修复愈合 ,其组织形态学变化可逆 ;未愈合者或病程大于 1 4天者 ,内耳形态学存在组织病理学改变 ,病理改变以及病程可作为手术干预的客观指标。
Objective: To establish an animal model to observe the effect of experimental perilymph fistula (EPLF) on the morphology of the inner ear and to provide experimental evidence for clinical treatment of the external lymphatic fistula. Methods: Twenty four guinea pigs were randomly divided into 6 groups with 4 animals in each group. The right ear was the control ear. A mini-syringe was used to puncture the left ear round window membrane (RWM) and extract 2 to 4 μl of perilymph to cause EPLF, which were sacrificed at 1, 2, 4, 7, 14 and 28 days after fistula. Electron microscope observation of inner ear morphological changes. Results: The majority of perforated RWMs (83%) were easy to heal. 2 to 4 days after rupture of RWM perforation at the organizational connection, 7 to 14 days basically healed. Scanning electron microscopy 4 days after fistula hair cell damage, fistula unhealed ear cochlear bottom hair cells were significantly reduced. CONCLUSIONS: There are two outcomes in EPLF: a single RWM perforation tends to heal spontaneously and its morphological changes are reversible. Histopathological changes in the morphology of the inner ear, pathological changes, and course of disease may be used as the surgical treatment for those who have not healed or have a duration of more than 14 days Objective indicators of intervention.