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目的 研究新发展晚期血吸虫病(晚血)患者循环mi RNAs的表达谱特征。方法 选取新发展晚血患者、未达到晚血的有史经治人群和健康对照人群各10例,采用Agilent Human micro RNAs microarray Rel 12.0芯片,检测570个人类相关的循环mi RNAs的表达水平,分析各组循环mi RNAs的表达差异。结果 与健康对照人群相比,新发展晚血患者的mi RNAs有4个表达上调,16个表达下调。而在健康对照和有史经治组的血清中存在着差异表达的mi RNAs共有27个,其中6个表达上调,21个表达下调。其中mi R-383在有史经治组和新发展晚血患者组中的表达分别降低了4.23倍和11.82倍。结论 新发展晚血患者、有史经治者和健康人群之间循环mi RNAs的表达特征存在显著差异,循环mi R-383可能与新发展晚血的发生发展有关,值得进一步研究。
Objective To study the expression profiles of circulating miRNAs in patients with newly developed advanced schistosomiasis (late blood). Methods Ten patients with newly developed late-onset blood and those without history of late-onset and healthy controls were enrolled in this study. The expression of 570 human circulatory mi RNAs was detected by Agilent Human micro RNAs microarray Rel 12.0 chip. Differences in expression of circulating miRNAs in groups. Results Compared with healthy controls, mi RNAs in newly developed late blood were up-regulated and down-regulated in 16 of them. There are 27 miRNAs differentially expressed in the serum of healthy controls and Shigejimei group, of which 6 were up-regulated and 21 were down-regulated. Among them, the expression of mi R-383 was decreased 4.23-fold and 11.82-fold respectively in the group of patients with history and the group of patients with newly developed late blood. Conclusions There are significant differences in the expression characteristics of circulating mi RNAs among newly developed patients with late-onset blood and those with and without history. The circulating mi-383 may be related to the development of late-onset blood and deserves further study.