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甲型肝炎病毒（HAV）是单股正链RNA病毒。HAV感染细胞脱壳后,RNA直接翻译为一个多蛋白,然后裂解为不同的结构和非结构蛋白,依赖病毒RNA的RNA聚合酶将基因组进行拷贝,产生由正链和负链RNA构成的增殖性中间体,负链又作为模板合成正链RNA,正链RNA装配上核壳成为新的病毒颗粒,而不再作为负链合成的模板。因此,负链RNA的量在HAV整个增殖周期中是非常低的,据估计不超过全部HAV RNA量的0.02％,但早期检测负链RNA,可以了解HAV的增殖,环境标本的HAV感染性,同时还可用于研究抗病毒物质在HAV生长
Hepatitis A virus (HAV) is a single-stranded positive-sense RNA virus. Following HAV infecting of cells, the RNA is directly translated into a polyprotein which is then cleaved to different structural and non-structural proteins. The viral RNA-dependent RNA polymerase copies the genome to produce a proliferative construct consisting of both positive and negative strands of RNA The intermediate, minus strand, also acts as a template for the synthesis of positive-stranded RNA, and positive strand RNA assembly on the nucleocapsid becomes a new virion rather than as a template for negative strand synthesis. Therefore, the amount of negative-stranded RNA is very low throughout the entire HAV cycle and is estimated to be no more than 0.02% of the total HAV RNA. However, early detection of negative-stranded RNA can reveal HAV proliferation, HAV infectivity in environmental samples, It can also be used to study the growth of antiviral substances in HAV