1987年我们着手香雪兰微繁殖技术的研究,用叶片、花序和胚繁殖均获得成功。香雪兰的体外组织再生能力很强。其主要再生途径有两种:1.诱导愈伤组织形成并诱导芽的分化(图1、2);2.从外植体的边缘直接产生芽(图3)。在第二种途径中,因没有愈伤组织形成阶段,大大减少了再生植株的变异率,也 In 1987, we started the study on the microspore propagation of Fraxenilla and succeeded in propagating the leaves, inflorescences and embryos. Fragrant in vitro tissue regeneration ability is very strong. There are two main ways to regenerate calluses: 1. Induction of callus formation and induction of shoot differentiation (Fig. 1, 2); 2. Direct generation of shoots from explant edges (Fig. 3). In the second approach, the mutation rate of regenerated plants is greatly reduced because there is no stage of callus formation