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探讨了１５和３０天尾吊法模拟失重对大鼠肿瘤坏死因子－α（ＴＮＦ－α）的含量及骨髓细胞对ＧＭ－ＣＳＦ反应性的影响。采用放射免疫分析方法检测腹腔巨噬细胞在ＬＰＳ刺激下产生ＴＮＦ－α的含量，３Ｈ－ＴｄＲ掺入法检测骨髓细胞对ＧＭ－ＣＳＦ的反应性。结果表明，模拟失重１５天后，ＴＮＦ－α含量有下降的趋势，３０天后ＴＮＦ－α含量显著减少（Ｐ＜０．０５）。１５天后，骨髓细胞对ＧＭ－ＣＳＦ的反应性显著下降（Ｐ＜０．０１），３０天后，两组间无明显改变。结果提示，１５及３０天模拟失重降低免疫功能，ＴＮＦ－α含量的减少可能与骨髓细胞的增殖功能有关。
The effects of simulated weightlessness on the levels of tumor necrosis factor-α (TNF-α) and the response of bone marrow cells to GM-CSF in 15 and 30 days tail-suspended method were investigated. The level of TNF-α produced by LPS stimulated by peritoneal macrophages was detected by radioimmunoassay. 3H-TdR incorporation was used to detect the reactivity of bone marrow cells to GM-CSF. The results showed that after 15 days of simulated weight loss, the content of TNF-α tended to decrease, and the content of TNF-α decreased significantly after 30 days (P <0.05). After 15 days, the reactivity of myeloid cells to GM-CSF was significantly decreased (P <0.01). After 30 days, there was no significant change between the two groups. The results suggest that 15 and 30 days simulated weightlessness reduce immune function, TNF-αlevels may be related to the proliferation of bone marrow cells.