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正常人血清和淋巴组织浆细胞中各IgG亚类所占比例分别为IgG_1 60%～65%,IgG_2 25%～30%,IgG_3 5%～10%,IgG_4≤5%;而在IgG~+B细胞中的比例为IgG_1≈40%,IgG_2≈50%,IgG_3≈8%,IgG_4≤5%,看来在B细胞分化的不同阶段调控IgG_1和IgG_2亚类的表达有差异.用正常人PBMC分离纯化T,B细胞.纯化的B细胞加PWM培养,并于其中分别加入PWM诱导的T细胞培养上清或不同组合的重组IL-2,IL-4,IL-6和IFN-γ,以测定各类淋巴因子对B细胞产生IgG亚类的影响;加入PHA刺激的T细胞于自体B细胞中测定T-B直接接触时对IgG产生的影响.
The percentage of IgG subclasses in normal plasma and lymphoid plasma cells were respectively IgG1 ~ 60% ~ 65%, IgG_2 ~ 25% ~ 30%, IgG_3 ~ 5% ~ 10%, IgG_4≤5% It seems that the expression of IgG1 and IgG2 subtypes is regulated at different stages of B cell differentiation by the ratio of IgG_1≈40%, IgG_2≈50%, IgG_3≈8%, and IgG_4≤5% Purified T and B cells Purified B cells were cultured in PWM, and then added with PWM-induced T cell culture supernatant or different combinations of recombinant IL-2, IL-4, IL-6 and IFN-γ The effect of various types of lymphokines on the production of IgG subclasses by B cells; the effect of adding PHA-stimulated T cells on IgG production in autologous B cells when direct TB exposure was detected.