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以带有腋芽的幼嫩土沉香茎段为外植体,运用DPS数据处理系统,通过均匀设计法筛选诱导土沉香茎段从出芽到生根整个再生体系的适宜培养基。结果表明:适宜土沉香茎段腋芽萌发的培养基为7/10MS+6-BA 0.35mg/L+NAA 0.01mg/L,诱导率为93.33%;适宜土沉香出芽增殖的培养基为7/10MS+KT 1.27mg/L+NAA 0.2mg/L,增殖倍数为3.53;适宜土沉香茎段生根的培养基为7/10MS+6-BA 0.02mg/L+IBA 1.23mg/L+CM 25mL/L,诱导率为33.33%。该试验优化了土沉香茎段再生体系的培养条件,为其快速繁殖的推广及遗传转化体系的建立提供了基础支持。
The stems and shoots of young soil with axillary buds were used as explants. The DPS data processing system was used to screen the suitable medium for inducing stem segments of Radix Rehmanniae Preparata from budding to rooting through the uniform design method. The results showed that the optimal medium for the induction of axillary buds was 7 / 10MS + 6-BA 0.35mg / L + NAA 0.01mg / L and the induction rate was 93.33% + KT 1.27mg / L + NAA 0.2mg / L, the proliferation multiplication was 3.53; suitable medium for the rooting of Radix Aconiti Kusnezoffii was 7 / 10MS + 6-BA 0.02mg / L + IBA 1.23mg / L + CM 25mL / L , The induction rate was 33.33%. The experiment optimized the culture conditions of stem regeneration system, provided the basic support for the promotion of rapid propagation and the establishment of genetic transformation system.