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将脲酶用戊二醛化学交联固定到单纤维尼龙网上,与氨气敏电极组合成结构简单的脲酶电极,此电极对脱有良好的响应特性,对脲浓度对数的响应线性范围为7×10(-5)~2×10(-2)kmol/m3,斜率为52mV,相关系数0.988,电极寿命大于20天,用于血清中脲的测量时样品不需要预处理,并与分光光度法所得结果有较好的一致性。
The urease was chemically crosslinked with glutaraldehyde to the monofilament nylon net and combined with the ammonia gas sensing electrode to form a simple urease electrode. The electrode had good response to delamination and had a linear response to the logarithm of urea concentration of 7 × 10 (-5) ~ 2 × 10 (-2) kmol / m3 with a slope of 52 mV and a correlation coefficient of 0.988, with an electrode life of more than 20 days. The sample used in serum urea measurement did not require pretreatment and was related to The results obtained by spectrophotometry have good agreement.