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目的:建立细辛药材不同部位GC指纹图谱及α-蒎烯、1、8-桉叶素、甲基丁香酚3种成分同时测定的定量分析方法。方法:利用毛细管气相色谱法对采集的6批细辛药材地上部分、根及根茎进行指纹谱分析;采用外标一点法,同时测定了来源于我国8个省34批细辛商品药材中α-蒎烯、1、8-桉叶素、甲基丁香酚含量。结果:以39个共有峰为指标,建立了细辛不同部位气相色谱指纹谱。GC法测定α-蒎烯、1、8-桉叶素、甲基丁香酚含量,进样量在2.58~46.44ng、2.292~32.088ng、3.7768~151.872ng范围内呈良好的线性关系,回归系数分别为0.9990、0.9990、0.9997,平均回收率为95.35%(RSD=2.38%)、96.11%(RSD=3.08%)、94.89%(RSD=2.71%)。结论:GC法建立的细辛药材不同部位指纹图谱及含量测定方法,可为细辛药材不同来源与不同部位的质量评价提供科学依据。
Objective: To establish a quantitative method for the simultaneous determination of GC fingerprints at different parts of Herba Asari and simultaneous determination of three components of α-pinene, 1,8-cineole, and methyl eugenol. METHODS: Fingerprint analysis was performed on the aboveground parts, roots and rhizomes of 6 batches of Asarum collected by capillary gas chromatography. A total of 34 batches of Asarum medicinal materials from 8 provinces in China were determined at the same time. Terpene, 1,8-cineole, methyl eugenol content. Results: Using 39 common peaks as indicators, gas chromatographic fingerprints of different parts of Asarum were established. The content of α-pinene, 1,8-cineole, and methyl eugenol was determined by GC. The linearity was well within the range of 2.58~46.44ng, 2.292~32.088ng, and 3.7768~151.872ng. They were 0.9990, 0.9990, and 0.9997, respectively, and the average recoveries were 95.35% (RSD=2.38%), 96.11% (RSD=3.08%), and 94.89% (RSD=2.71%). Conclusion: The fingerprints and determination methods of different parts of Herba Asari which were established by GC method can provide scientific basis for quality evaluation of different sources and different parts of Asarum.