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本文作者对杜氏利什曼和巴西利什曼鞭毛体在培养基中需要的营养进行了研究,两种利什曼鞭毛体在加有氯化钠和氯化钙等多种无机盐、14种L-氨基酸(精氨酸、半胱氨酸、谷酰胺、组氨酸、异白氨酸、亮氨酸、赖氨酸、旦氨酸、苯基丙氨酸、脯氨酸、苏氨酸、色氨酸、酪氨酸、缬氨酸)、葡萄糖、腺甙及11种维生素与有关生长因子的混合物的培养基中能连续生长繁殖传代。在培养基内加以纯净去脂的牛血清蛋白对两种利什曼鞭毛体生长繁殖也有利。两种利什曼原虫需要的营养与
The authors studied the nutrient requirements of Leishmania and Brazilian Leishmania in culture medium. Two types of Leishmanian flagella were treated with various inorganic salts such as sodium chloride and calcium chloride, and 14 L-amino acids (arginine, cysteine, glutamine, histidine, isoleucine, leucine, lysine, tryptophan, phenylalanine, proline, threonine , Tryptophan, tyrosine, valine), glucose, adenosine, and a mixture of 11 vitamins and related growth factors. Pure lean bovine serum albumin in culture medium is also beneficial for the growth and reproduction of both Leishman’s flagellar bodies. Two kinds of Leishmania need nutrition and