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目的:观察地尔硫芯卓对实验家兔动脉粥样硬化模型的干预作用,并探讨钙拮抗剂抗动脉粥样硬化的作用机制。方法:36只大白兔随机分为4组,正常组对照组、高脂对照组、阿托伐他汀组、地尔硫芯卓组,每组9只。观察实验后家兔血总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL)、白细胞介素6(IL-6)、C反应蛋白(CRP)在各组间的含量变化,测定实验结束时家兔主动脉粥样硬化斑块的面积及显微镜下病理形态表现。结果:与高脂组相比,阿托伐他汀组TC、TG、LDL-C、IL-6、CRP含量明显降低(P<0.01),主动脉粥样硬化面积明显降低(P<0.01);与高脂组相比,地尔硫芯卓组TC、LDL-C、IL-6、CRP降低(P<0.01),主动脉粥样硬化面积明显降低(P<0.01);与阿托伐他汀组相比,地尔硫芯卓组IL-6明显降低(P<0.01),CRP、TC、TG明显升高(P<0.01)。结论:钙拮抗剂地尔硫芯卓对动脉粥样硬化有明显的预防和治疗作用,其机制可能和抗氧化、减少炎性介质IL-6、CRP的释放、改善内皮细胞功能有关。
Objective: To observe the intervention of Diltiazem on atherosclerosis model in rabbits and to explore the mechanism of calcium antagonist on atherosclerosis. Methods: Thirty - six rabbits were randomly divided into 4 groups: normal control group, high fat control group, atorvastatin group and diltiazem group. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL), interleukin 6 (IL-6), C-reactive protein (CRP) content in each group, to determine the end of the experiment rabbit aortic atherosclerotic plaque area and microscopic pathological findings. Results: The levels of TC, TG, LDL-C, IL-6 and CRP in atorvastatin group were significantly decreased (P <0.01) and the area of aortic atherosclerosis was significantly decreased (P <0.01). The LDL-C, IL-6 and CRP levels in the diltiazem group were significantly lower than those in the high-fat diet group (P <0.01) and the aortic atherosclerosis area was significantly decreased (P <0.01) Compared with the control group, IL-6 in diltiazem group was significantly lower (P <0.01) and CRP, TC and TG were significantly increased (P <0.01). CONCLUSION: Diltiazem, a calcium antagonist, has a significant preventive and therapeutic effect on atherosclerosis. Its mechanism may be related to the anti-oxidation, reducing the release of IL-6 and CRP in inflammatory mediators and improving the function of endothelial cells.