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目的 探讨亚氨乙基赖氨酸对豚鼠噪声性损伤的拮抗作用。方法 选健康白色红目豚鼠 4 0只 ,随机分为 4组 :A组为正常对照组 ,B组为噪声组 ,C组为噪声 +药物组 ,D组为亚氨乙基赖氨酸组。B、C组豚鼠暴露于 115dB白噪声连续 6d ,每天连续 6h ;C组豚鼠每天腹腔注射亚氨乙基赖氨酸 10mg/kg ,B组豚鼠腹腔注射等量的生理盐水 ,D组豚鼠每天腹腔注射亚氨乙基赖氨酸 10mg/kg,并不暴露于噪声。各组豚鼠在实验前、后均行ABR听阈检测。用免疫组化方法检测诱生型一氧化氮合成酶 (NOSⅡ )在各组豚鼠耳蜗的表达。各组豚鼠耳蜗行扫描电镜检查。观察比较各组豚鼠ABR听阈、NOSⅡ染色强弱及耳蜗形态。结果 实验前 ,A、B、C、D各组间ABR听阈的差异无显著性 (P >0 .0 5 )。实验后 ,A组、D组ABR听阈无明显改变 ,而B组和C组ABR听阈则有明显改变 ,B组ABR听阈为 (6 0 .2 3± 11.2 3)dB ,C组为 (38.4 6± 7.2 4 )dB ,两组比较 ,差异有显著性 (P <0 .0 5 )。NOSⅡ在A、D组耳蜗表达阴性 ,B组耳蜗表达较强 ,C组耳蜗表达较弱。B组豚鼠耳蜗外毛细胞损伤较C组重。结论 NOSⅡ在噪声所致豚鼠耳蜗损伤中呈阳性表达 ,亚氨乙基赖氨酸能抑制NOSⅡ的活力 ,且对噪声所致豚鼠耳蜗损伤有拮抗作用 ,表明一氧化氮在噪声性聋的发病中起重要作用。
Objective To investigate the antagonistic effect of iminoethlysine on noise-induced injury in guinea pigs. Methods Forty healthy red guinea pigs were randomly divided into four groups: group A was normal control group, group B was noise group, group C was noise + drug group, group D was iminoethyl lysine group. B and C guinea pigs were exposed to 115dB white noise for 6 days continuously for 6 hours every day. C group guinea pigs were injected intraperitoneally with 10 mg / kg iminolysine lysine, group B guinea pigs were injected intraperitoneally with normal saline, Injections of iminoethyllysine 10 mg / kg were not exposed to noise. The guinea pigs in each group before and after the test were ABR hearing threshold test. Immunohistochemistry was used to detect the expression of inducible nitric oxide synthase (NOSⅡ) in guinea pig cochlea. The cochlea of each group were examined by scanning electron microscopy. The ABR threshold, the intensity of NOS Ⅱ staining and the morphology of cochlea in guinea pigs of each group were observed and compared. Results Before the experiment, there was no significant difference in ABR hearing threshold between groups A, B, C and D (P> 0.05). After the experiment, there was no significant change of ABR threshold in group A and group D, but the ABR threshold of group B and group C was significantly changed. The ABR threshold of group B was (60.2 ± 11.23) dB, and group C was (38.4 6) ± 7.2 4) dB, the difference between the two groups was significant (P <0.05). NOSⅡwas negative in cochlear in group A and group D, while cochlear in group B was stronger than that in group C, and cochlear in group C was weaker. B group of guinea pig cochlear outer hair cell damage than C group weight. Conclusion NOSⅡ is positively expressed in noise-induced guinea pig cochlea injury. Imino-lysine can inhibit the activity of NOSⅡ and antagonize the noise-induced cochlear damage in guinea pigs, indicating that nitric oxide is involved in the pathogenesis of noise-induced deafness makes an important impact.