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作者将激发B细胞、辅助性T(Th)细胞和细胞毒性T淋巴细胞(CTL)应答的三个表位的寡核苷酸(HA91-108、NP55-69和NP147-158),分别插入沙门菌鞭毛蛋白基因中,得到杂交鞭毛蛋白Fla-55、Fla-147和Fla-91.将其免疫BALB/c小鼠,检测特异性体液免疫和细胞免疫应答.为评估疫苗诱导长期记忆和保护作用的能力,作者分别用Fla-对照、Fla-91+Fla-147和Fla-55+Fla-91+Fla-147鼻内免疫小鼠3次,间隔3周.末次免疫后1、4和7个月,用流感病毒A/得克萨斯/77攻击.结果表明,合成疫苗诱导的保护性免疫力至少可持续7个月.含有三种表位的疫苗免疫效果最好.Th表位与另两种表位协同时,可明显增强保护作用.所有A/H3株都有HA91-108,所有甲型病毒都具有核蛋白.因此有必要确定这些表位疫苗能否诱导交叉保护.结果表明,小鼠免疫后1个月,用甲型流感病毒株(两株为H3N2亚型,1株为H2N2型)攻击,接种Fla-91疫苗的小鼠仅能抵抗H3亚型病毒,增加Fla-147能增强Fla-91的保护力,对H2株也
The authors inserted oligonucleotides (HA91-108, NP55-69 and NP147-158) that excitated the three epitopes of B cells, helper T (Th) cells and cytotoxic T lymphocyte (CTL) responses into the Salmonella The flagellin Fla-55, Fla-147 and Fla-91 were obtained from the bacterial flagellin gene, which were then used to immunize BALB / c mice to detect specific humoral and cellular immune responses To evaluate the long-term memory and protection induced by the vaccine Mice were immunized intranasally three times with Fla-control, Fla-91 + Fla-147 and Fla-55 + Fla-91 + Fla-147 at intervals of 3 weeks, 1, 4 and 7 post-immunization Month and challenged with influenza A / Texas / 77.The results showed that the protective immunity induced by the synthetic vaccine lasted for at least 7 months.The vaccines containing the three epitopes had the best immunization effect.Th epitopes and the other two forms Bit synergy, can significantly enhance the protection of all A / H3 strains have HA91-108, all viruses have a nuclear protein nucleotides Therefore, it is necessary to determine whether these epitope vaccines can induce cross protection The results show that the mice immunized One month after the challenge, influenza A virus strains (two strains of H3N2 and one strain of H2N2) were challenged, and mice vaccinated with Fla-91 vaccine only showed resistance to H3 Subtypes of the virus, increased Fla-147 can enhance the protective effect of Fla-91 on the H2 strain also