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经能量为２０～３０Ｋｅｖ注入剂量为６×１０１５Ｎ＋／ｃｍ２和１０×１０１５Ｎ＋／ｃｍ２的Ｎ＋离子注入及免疫荧光抗体标记显微观察和统计，结果表明，在绿豆根尖细胞有丝分裂中，分裂细胞的形体、中后期细胞数目、细胞微管骨架以及纺缍体的排布方式均发生了异常变化。１２－１３％的分裂细胞个体增大，８－１０％有丝分裂中后期细胞数目高于对照组，间期细胞微管骨架荧光强度明显弱于对照组，且有２－４％的较强荧光小体和无荧光小体（微孔洞，ｍｉｃｒｏｏｐｅｎｉｎｇ）。３－１２％的中后期细胞中出现不对称和极向不同步移动排布的纺缍体。研究结果表明：２０～３０Ｋｅｖ的能量和剂量为６～１０×１０１５Ｎ＋／ｃｍ２的Ｎ＋离子束注入，对绿豆根尖细胞有丝分裂微管骨架可诱发产生异常变化，同时也可以初步认为这种变化结果与Ｎ＋离子束注入产生的染色体异常分配和运动功能活动有密切的相关性。
The results showed that in the mitosis of mung bean root tip cells, the morphology of the dividing cells was marked by N + ion implantation and immunofluorescence antibody labeling with the dose of 6 × 10 15 N + / cm 2 and 10 × 10 15 N + / cm 2 with the energy of 20-30 Kev. , The number of cells in mid and late, the cell microtubule skeleton and the arrangement of spunlaced body abnormal changes have taken place. The number of mitotic cells increased from 12% to 13%, and the number of cells in 8-10% late mitosis was higher than that in control group. The fluorescence intensity of interphase microtubule cytoskeleton was significantly weaker than that of control group, and 2-4% Body and non-fluorescent bodies (micro-holes). 3-12% of the cells in the late emergence of asymmetric and the direction of movement of the unsynchronized arrangement of spinning body. The results show that 20 ~ 30Kev energy and dose of 6 ~ 10 × 1015N + / cm2 of N + ion beam implantation, mung bean root tip cell mitotic microtubule skeleton can induce abnormal changes, but also can be initially considered that the results of this change and There is a close correlation between abnormal distribution of chromosomal aberrations and motility function produced by N + ion beam implantation.