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目的通过参加CNAS(中国合格评定国家认可委员会)食品中副溶血性弧菌检测能力验证计划,对实验室检测检测能力及质量进行确认。方法采用国标法及实时荧光PCR法对标识为VP-162考核样品及超市随机抽取的50份动物性海水产品进行副溶血性弧菌定性检测,通过检测结果比较进行方法评价。结果 VP-162考核样品中检出副溶血性弧菌,考核结果为满意。随机抽取的50份动物性海水产品,干制生化试剂及API20E均检出6份(检出率12.0%),全自动微生物生化鉴定系统检出5份(检出率10.0%),实时荧光PCR法检出8份(检出率16.0%)。结论实时荧光PCR检测结果准确、灵敏、快速,在运用国标法进行检测过程中,采用生化鉴定试剂与全自动微生物生化鉴定系统分别进行鉴定时,其鉴定结果存在一定差异。
Objective To confirm the ability and quality of laboratory testing and testing by participating in CNAS (National Accreditation Board for Conformity Assessment of China) food Vibrio parahaemolyticus testing proficiency testing program. Methods Fifty samples of animal seawater products randomly selected from VP-162 assessment samples and supermarket were qualitatively tested by GB method and real-time fluorescence PCR method. The method was compared by the test results. Results Vibrio parahaemolyticus was detected in VP-162 test sample, and the test results were satisfactory. Six of the 50 randomly selected samples of animal seawater products, dried biochemical reagents and API20E were detected (detection rate was 12.0%), five were detected by the automatic microbial biochemical identification system (detection rate was 10.0%), real-time fluorescence PCR Act detected 8 (detection rate of 16.0%). Conclusion The results of real-time PCR are accurate, sensitive and rapid. In the process of using national standard method to carry out the detection, the results of biochemical identification reagents and automatic microbial biochemical identification systems are different.