论文部分内容阅读
为体外培养角膜上皮移植选取细胞来源，本文应用溴代脱氧尿嘧啶（Ｂｒｄｕ，胸腺嘧啶的类似物）渗入ＤＮＡ合成，抗—Ｂｒｄｕ单克隆荧光抗体标记细胞法，在流式细胞仪（ＦＡＣＳｔａｒｐｌｕｓ）上检测离体情况下兔角膜不同区域上皮细胞对５－ＦＵ耐受情况，以确定长周期细胞的部位。结果显示：经５－ＦＵ处理后的角膜周边及角膜中央部上皮细胞增殖明显受到抑制，而角膜缘细胞对５－ＦＵ毒性则有相对耐受性。由此得出：角膜缘上皮细胞群中有长周期的角膜干细胞存在。因而，行体外培养角膜上皮时，应选取角膜缘部的细胞。
In order to select the cell source for corneal epithelial transplantation in vitro, we used BrdU (thymine analogue) to penetrate into DNA synthesis and anti-Brdu monoclonal antibody fluorescent labeled cell method. On the FACStarplus To examine the tolerance of epithelial cells to 5-FU in different regions of rabbit cornea in vitro to determine the location of long-period cells. The results showed that 5-FU-treated corneal epithelium and central corneal epithelium significantly inhibited the proliferation of epithelial cells, whereas limbal cells were relatively tolerant to 5-FU toxicity. It follows that there are long-term keratocytes in the limbal epithelial cell population. Therefore, in vitro culture of corneal epithelium, corneal limbal cells should be selected.