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目的：探讨牛视网膜提取物对人－鼠杂合细胞抗体产生的影响。方法：在ＰＲＭＩ１６４０完全培养基（ＣＭ）中加入２０ｍｇ／Ｌ牛视网膜提取蛋白，配成牛视网膜培养基（ＢＲＥＭ）。用ＭＴＴ测定、细胞克隆技术、夹心ＥＬＩＳＡ方法和染色体分析，比较人－鼠杂合细胞Ｇ１２在两种培养条件下的细胞活力、克隆效率、人ＩｇＭ分泌和人染色体阳性细胞比率之间的差异。结果：用ＢＲＥＭ培养的Ｇ１２细胞活力高于用ＣＭ培养（Ｐ＜００５）。用ＢＲＥＭ培养的Ｇ１２细胞克隆中分泌人ＩｇＭ者占１２／２４，用ＣＭ培养的克隆只占２／４７。Ｇ１２细胞传代培养３个月后，用ＢＲＥＭ培养的上清人ＩｇＭ水平Ａ４９０为０３３５±００５０，用ＣＭ培养的上清为００７０±００２７（Ｐ＜００５）。前者含人染色体阳性的细胞为２０％，后者２５％，两者间没有差异（Ｐ＞００５）。结论：添加牛视网膜提取物的培养基可以提高人－鼠杂合细胞的活力和人ＩｇＭ抗体的分泌能力。维持人源性ＩｇＭ持续分泌的条件不仅取决于杂合细胞中人染色体的存在和稳定，还有其它未知因素在起作用，牛视网提取蛋白可以提供这方面的需要。
Objective: To investigate the effects of bovine retinal extracts on the production of human-mouse hybrid cells. METHODS: 20 mg/L bovine retinal protein was added to PRMI1640 complete medium (CM) to prepare bovine retinal medium (BREM). Using MTT assay, cell cloning technique, sandwich ELISA method and chromosome analysis, the difference in cell viability, cloning efficiency, human IgM secretion, and human chromosome positive cell ratio of human-murine hybrid cell G12 under two culture conditions was compared. Results: The viability of G12 cells cultured with BREM was higher than that with CM (P < 0.05). Of the G12 cell clones cultured with BREM, 12/24 secreted human IgM, and clones cultured with CM accounted for only 2/47. After G12 cells were subcultured for 3 months, the A490 level of A490 in the supernatants cultured with BREM was 0335±0050, and the supernatant in CM culture was 0070±0027 (P<005). The former contained 20% of chromosome-positive cells and 25% of the latter, with no difference between the two (P>005). CONCLUSION: The medium supplemented with bovine retinal extract can increase the viability of human-murine hybrid cells and the secretion of human IgM antibody. Maintaining the conditions for sustained secretion of human IgM depends not only on the presence and stability of human chromosomes in hybrid cells, but also on the role of other unknown factors in the extraction of bovine reticulin.