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目的:建立广藿香HPLC指纹图谱的分析方法,对四个主要产地的广藿香指纹图谱进行比较,为广藿香药材内在质量评价积累数据。方法:色谱柱:ODS色谱柱Diamonsil(R)C18(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸水溶液梯度洗脱;检测波长:254nm;柱温:25℃;流速:1.0mL/min。结果:四个不同产地的广藿香指纹图谱存在一定的差异,其中广州黄村和肇庆高要的药材相似度大于0.9,而这两地与湛江吴川和海南万宁比较差异较大。结论:方法准确可靠,重复性好,为广藿香药材道地性、真伪鉴别及品质评价提供了参考依据。
OBJECTIVE: To establish an HPLC fingerprinting method for patchouli and compare the fingerprints of patchouli in four major areas of origin, accumulating data for evaluating the intrinsic quality of patchouli herbs. Method: Column: ODS column Diamonsil (R) C18 (250 mm × 4.6 mm, 5 μm); mobile phase: gradient elution with methanol-0.1% phosphoric acid; detection wavelength: 254 nm; mL / min. Results: There were some differences in the fingerprints of patchouli in four different habitats. The similarities of the herbs in Huangcun and Zhaojing of Guangzhou were greater than 0.9, but the differences between them were quite different from those in Wuchuan and Zhanjiang. Conclusion: The method is accurate, reliable and reproducible. It provides a reference for the authenticity, authenticity and quality evaluation of patchouli herbs.