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目的:探讨前列腺I号水丸对慢性细菌性前列腺炎(CMP)大鼠组织内菌落数量、一氧化氮(NO)浓度和一氧化氮合酶(NOS)活力的影响。方法:健康成年雄性Wistar大鼠120只,体重250~300 g,随机分为6组,分别为空白对照组、模型对照组、阳性对照组、水丸高剂量组、水丸中剂量组、水丸低剂量组,每组20只。前列腺注射大肠杆菌(107个/ml)建立CMP大鼠动物模型。造模成功1个月后,用前列腺水丸高[4.4 g/(kg.day)]、中[2.2 g/(kg.day)]、低[1.1 g/(kg.day)]剂量灌胃治疗,阳性对照组用美满霉素[0.018 g/(kg.day)]灌胃,模型组和空白组用生理盐水灌胃。连续治疗35 d后,计算大鼠前列腺组织匀浆菌落数。采用亚硝酸盐还原酶法检测前列腺组织中NO变化并计算NOS活力的变化。结果:前列腺Ⅰ号水丸高、中、低剂量组大鼠组织内菌落数的水平和前列腺组织内NO含量及NOS活力明显低于模型组,与模型组比较差异显著(P<0.01)。结论:前列腺Ⅰ号水丸对慢性细菌性前列腺炎模型大鼠有一定治疗作用。其作用机制可能与抑制前列腺内细菌生长以及降低前列腺组织内NO含量和NOS活力有关。
Objective: To investigate the effects of Prostate I waterball on the numbers of colony, nitric oxide (NO) and nitric oxide synthase (NOS) in rats with chronic bacterial prostatitis (CMP). Methods: 120 healthy adult male Wistar rats weighing 250-300 g were randomly divided into 6 groups: control group, model control group, positive control group, high dose group of water pill, medium dose group of water pill and low dose of water pill Group, 20 in each group. Prostate injection of Escherichia coli (107 / ml) to establish a rat model of CMP. After 1 month of successful modeling, intragastric administration of Prostate Pills [4.4 g / (kg.day)], [2.2 g / (kg.day)], low [1.1 g / (kg.day)] , The positive control group with minocycline [0.018 g / (kg.day)] intragastric administration, the model group and the blank group with saline. After 35 days of continuous treatment, the number of colonies in the rat prostate homogenates was calculated. Nitrite reductase was used to detect the change of NO in prostatic tissue and calculate the change of NOS activity. Results: The number of colonies and the content of NO and the activity of NOS in prostate tissue were significantly lower than those in model group (P <0.01). Conclusion: Prostate Ⅰ water pill has a certain therapeutic effect on rats with chronic bacterial prostatitis. Its mechanism may be related to the inhibition of bacterial growth in the prostate and the reduction of NO content and NOS activity in prostate tissue.