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本文报告以自制玻璃冷却小壶为主的冷凝装置在-21℃下连续收集测定呼出气微量氚水的方法。由腔静脉“团块”注入氚水,每5秒呼出气为一样本,测定1min 正常兔呼出气氚水浓度曲线。18只兔33次实验的平均曲线中,第一个样本氚水浓度1.16±0.31(SE),继而迅速上升,25—40s 达最高值(10.43—10.53),以后缓慢下降,平均浓度7.93±0.47(SE)。阻断支气管动脉时呼出气氚水浓度为未阻断时的65%±15%(SD);主动脉弓注入氚水的模拟再循环呼出气氚水浓度为对照值的4.31%;呼出气氚水平均浓度与通气指标、体温、环境湿度不相关。结果说明呼出气氚水浓度曲线是氚水经肺首次循环时透过血-气屏障的转移所产生。呼出气氚水曲线高峰滞后于血液稀释曲线高峰20—35(?)s,可能反映氚水在肺内转移中有滞留现象.呼出气氚水浓度曲线可作为研究肺水转移和诊断肺水肿的新方法,我们首次观察的现象和数据亦可作进一步研究的参考
This report reports the method of continuous determination of trace amounts of tritiated water in expired air at -21 ° C by a condensing unit such as a self-made glass cooling canister. By the cava “clumps” into the tritiated water exhaled every 5 seconds for a sample, measured 1min normal rabbit exhaled gas tritiated water concentration curve. In the mean curve of 33 experiments of 18 rabbits, the tritiated water concentration of the first sample was 1.16 ± 0.31 (SE), then rose rapidly and reached the highest value (10.43-10.53) at 25-40s, then decreased slowly with an average concentration of 7.93 ± 0.47 (SE). When the bronchial artery was occluded, the tritium concentration in exhaled breath was 65% ± 15% (SD) when unobstructed; the simulated recirculation exhaled tritium concentration in the aortic arch was 4.31% of the control value; Concentration and ventilation indicators, body temperature, humidity is not related. The result shows that the curve of exhaled gas tritium concentration is generated through the transfer of blood-air barrier when the tritiated water is first circulated through the lung. The peak of exhaled breath tritiated water lags behind the peak of hemodilution curve for 20-35 (?) S, which may indicate the retention of tritiated water in intrapulmonary metastasis. The curve of exhaled breath of tritiated water can be used to study lung water transfer and diagnosis of pulmonary edema The new method, our first observation of the phenomenon and the data can also be used for further research reference