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在小鼠右腋皮下接种Ｈ２２肿瘤细胞悬液，灌服含硒液（硒酸酯多糖）有抑制皮下肿瘤块生长的作用，在人肝癌细胞株培养液中，加入一定浓度含硒液（２０μｇ／ｍｌ、５０μｇ／ｍｌ、１５０μｇ／ｍｌ），能抑制肝癌细胞增殖，并促使细胞凋亡。与此同时，小鼠血液中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性提高，血浆和细胞培养液中ＮＯ含量升高。探讨硒酸脂多糖引起肝癌细胞凋亡，可能与使抗氧化活性提高、阻遏自由基对癌细胞增殖的介导及促使ＮＯ释放增加有关。
H22 tumor cell suspension was inoculated subcutaneously in the right iliac crest of mice, and selenium-containing liquid (selenate polysaccharide) was used to inhibit the growth of subcutaneous tumor mass. In the culture solution of human hepatoma cell line, a certain concentration of selenium-containing solution (20 μg) was added. /ml, 50 μg/ml, 150 μg/ml) can inhibit the proliferation of hepatoma cells and promote apoptosis. At the same time, the activity of glutathione peroxidase (GSH-Px) in the blood of mice increased, and the levels of NO in plasma and cell culture fluids increased. To investigate the apoptosis of hepatocellular carcinoma cells induced by selenite lipopolysaccharide may be related to the increase of antioxidative activity, inhibition of free radicals on proliferation of cancer cells and promotion of increased NO release.