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[目的]观察孕前慢性应激后仔鼠学习记忆能力与海马中胰岛素样生长因子Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)的表达变化,探讨仔鼠学习记忆能力变化的机制。[方法]建立慢性应激孕前大鼠模型,通过血浆皮质酮动态测定来检测模型,采用Morris水迷宫和Y迷宫进行学习记忆能力测定、实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)检测海马中IGF-Ⅱ表达水平。[结果]模型组母鼠血浆皮质酮水平增高(P<0.05);模型组所产仔鼠只数及母鼠孕天数均少于对照组(P<0.05),两组雌雄比例差异无统计学意义(P>0.05);模型组仔鼠血浆皮质酮高于对照组仔鼠(P<0.05);模型组仔鼠学习、记忆能力低于对照组(P<0.05);模型组仔鼠IGF-ⅡmRNA表达量低于对照组仔鼠(P<0.05)。[结论]孕前慢性应激后仔鼠空间学习记忆能力下降,可能与其脑内IGF-Ⅱ表达的下调及体内皮质酮升高有关系。
[Objective] To observe the learning and memory abilities and the expression of insulin-like growth factor-Ⅱ (IGF-Ⅱ) in the hippocampus of young rats after preconception and to explore the mechanism of learning and memory abilities of offspring. [Methods] Chronic stress preconception rat model was established. The plasma corticosterone level was used to detect the model. Morris water maze and Y maze were used to measure learning and memory ability. Real-time polymerase chain reaction , RT-PCR) to detect the expression of IGF-Ⅱ in hippocampus. [Results] The level of plasma corticosterone in the model group was significantly higher than that in the control group (P <0.05). The number of offspring born in the model group and the number of maternal pregnant days in the model group were all less than those in the control group (P <0.05) (P <0.05). The plasma corticosterone in model group was higher than that in control group (P <0.05). The learning and memory abilities in model group were lower than those in control group (P <0.05) Ⅱ mRNA expression was lower than the control group pups (P <0.05). [Conclusion] Decreased spatial learning and memory abilities in premature newborn rats may be related to the down-regulation of IGF-II expression and the increase of corticosterone in the brain.