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目的应用HPLC,建立同时测定痹可通散中升麻素苷、5-O-甲基维斯阿米醇苷和苍术素含量的方法。方法 Inertsil ODS-3色谱柱(250 mm×4.6 mm,5μm),以甲醇-乙腈(体积比为2∶1)为流动相A,以体积分数为0.1%的磷酸水溶液为流动相B,梯度洗脱,检测波长:300 nm,流速:1.0 ml·min~(-1),柱温:30℃;进样量:20μL。结果升麻素苷、5-O-甲基维斯阿米醇苷和苍术素的线性分别为10.00~60.00、10.00~60.00、14.00~84.00 mg·L~(-1),相关系数分别为0.999 7、0.999 6、0.999 6,平均回收率分别为98.7%、98.1%、97.7%,RSD分别为1.7%、1.6%、1.5%(n=6)。结论所建立的方法为痹可通散的质量标准提供实验依据。
Objective To establish a method for simultaneous determination of ciclinoid, 5-O-methylvishisainin and atractylodes in BiKetong San by HPLC. Methods Inertsil ODS-3 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase A consisted of methanol and acetonitrile (2: 1 by volume) as mobile phase A and the mobile phase B consisted of 0.1% Off, detection wavelength: 300 nm, flow rate: 1.0 ml · min -1, column temperature: 30 ℃; injection volume: 20 μL. Results The linearities of cimnil, 5-O-methylvishisainin and atractylodes were 10.00 ~ 60.00, 10.00 ~ 60.00, 14.00 ~ 84.00 mg · L -1, the correlation coefficients were 0.999 7,0.999 6,0.999 6. The average recoveries were 98.7%, 98.1% and 97.7%, respectively, with RSDs of 1.7%, 1.6% and 1.5%, respectively (n = 6). Conclusion The established method provides an experimental basis for the quality standard of Bi Tong Tong San.