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采用~(125)IUdR释放法测定了不同浓度的枸杞子多糖、黄芪多糖、刺五加多糖及鼠伤寒杆菌内毒素多糖对不同剂量rIL-2激活的杀伤细胞(LAK)抗肿瘤活性的影响,发现4种多糖在一定浓度条件下均可在体外增强LAK活性。枸杞子多糖在0.01～0.1mg·ml~(-1)、黄芪多糖和刺五加多糖在0.01mg·ml~(-1)时增强作用最显著(P<0.001),内毒素多糖在0.0001～0.1μg·ml~(-1)时可增强LAK活性,在0.0001μg·ml~(-1)时最显著。4种多糖浓度过低或过高则不能增强,甚至抑制LAK活性。
The effects of different concentrations of Gardenia polysaccharides, polysaccharides from Astragalus, Acanthopanax senticosus polysaccharides and endotoxin polysaccharides from Salmonella typhimurium on the antitumor activity of different doses of rIL-2 activated killer cells (LAK) were determined by ~(125)IUdR release method. It was found that the four polysaccharides can enhance LAK activity in vitro under certain concentration conditions. The polysaccharides in Gardenia jasminoides had the most significant enhancement effect (0.01~0.1 mg·ml~(-1)), astragalus polysaccharides and Acanthopanax senticosus polysaccharide at 0.01 mg·ml -1 (P<0.001), and the endotoxin polysaccharides were in the range of 0.0001～. The activity of LAK was increased at 0.1 μg·ml -1 , which was most significant at 0.0001 μg·ml -1. If the concentration of the four polysaccharides is too low or too high, they cannot be enhanced, and even LAK activity is inhibited.