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目的 研究左旋多巴对黑质细胞的神经毒性作用 ,探讨合理应用左旋多巴治疗帕金森病的方法。方法 通过 6-羟基多巴胺脑立体定向注射术制作大鼠帕金森病模型 ,采用TUNEL方法观察左旋多巴小(每天每公斤 10mg)、中 (每天每公斤 5 0mg)、大 (每天每公斤 10 0mg)三种不同剂量、不同的作用时间 (1d、3d、5d、7d)对帕金森病大鼠黑质细胞的毒性作用 ,并观察治疗后 7d各项指标的变化。结果 帕金森病大鼠细胞凋亡数随着左旋多巴治疗的时间、剂量增加而增加。结论 左旋多巴能加速帕金森病大鼠黑质细胞凋亡 ,小剂量、间隔使用左旋多巴能有效减少其神经毒性作用
Objective To study the neurotoxic effects of levodopa on the substantia nigra cells and to explore the rational use of levodopa in the treatment of Parkinson’s disease. Methods Parkinson’s disease model was induced by 6-hydroxydopamine stereotactic injection. TUNEL method was used to observe the effects of levodopa (10 mg / kg / day), medium (50 mg / kg / day), large ) Three kinds of different doses and different action time (1d, 3d, 5d, 7d) on the toxicity of Parkinson’s disease in rat substantia nigra and observed the change of each index 7d after treatment. Results The number of apoptotic cells in Parkinson’s disease rats increased with the dose of levodopa treatment. Conclusion Levodopa can accelerate the apoptosis of nigral cells in rats with Parkinson’s disease. Low dose and interval levodopa can effectively reduce the neurotoxicity