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目的通过介导过氧化物酶体增殖剂激活受体(PPARs)探讨邻苯二甲酸二乙基己酯(DEHP)对雌性生殖系统影响。方法将40只21 d雌性ICR小鼠随机分成DEHP 0、20、100、500 mg/kg剂量组,连续染毒10 d,停止染毒24 h后处死动物,用实时聚合酶链式反应(real time-PCR)技术测定小鼠PPARs mRNA表达水平,用酶联免疫吸附法(ELISA)检测小鼠血清中雌二醇、孕酮水平。结果 100、500 mg/kg DEHP组卵巢湿重分别为(0.01±0.003)、(0.01±0.007)g,脏器系数分别为(0.13±0.02)%、(0.12±0.03)%,与对照组比较,明显降低(P<0.05);500 mg/kg DEHP组小鼠血清中雌二醇水平明显低于对照组(P<0.05);各染毒组小鼠血清孕酮水平与对照组之间无明显差异(P>0.05);与对照组比较,各染毒组PPARα、PPARβmRNA表达水平无明显变化(P>0.05),PPARγmRNA表达水平明显升高(P<0.05)。结论 DEHP作为一种过氧化物酶体增殖剂可以通过改变PPARs基因表达水平影响雌性小鼠卵巢功能。
Objective To investigate the effect of diethylhexyl phthalate (DEHP) on female reproductive system by mediating peroxisome proliferator-activated receptors (PPARs). Methods Forty-two female ICR mice, 21 days old, were randomly divided into DEHP 0, 20, 100, and 500 mg / kg dose groups for 10 days. Animals were sacrificed 24 hours after exposure and real-time polymerase chain reaction time-PCR technique was used to detect the mRNA expression of PPARs in mice. Serum estradiol and progesterone levels were detected by enzyme-linked immunosorbent assay (ELISA). Results The ovarian wet weights in the 100 and 500 mg / kg DEHP groups were (0.01 ± 0.003) and (0.01 ± 0.007) g, respectively. The organ coefficients were 0.13 ± 0.02% and 0.12 ± 0.03%, respectively. Compared with the control group (P <0.05). The serum estradiol level of 500 mg / kg DEHP group was significantly lower than that of the control group (P <0.05). The serum progesterone level of mice in each exposure group was significantly lower than that of the control group (P> 0.05). Compared with the control group, the expression levels of PPARα and PPARβmRNA did not change significantly (P> 0.05) and PPARγmRNA expression levels significantly increased (P <0.05). Conclusion DEHP as a peroxisome proliferator can affect the ovarian function of female mice by changing the PPARs gene expression level.