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以同基因型葡萄的四个脯氨酸累积变异系为材料，研究了不同浓度ＮａＣｌ胁迫下葡萄脯氨酸累积变异系的耐盐性。结果表明，葡萄脯氨酸累积变异系对高浓度ＮａＣｌ胁迫有较强抗性。在２４０ｍｍｏｌ·Ｌ－１胁迫下，对照系愈伤组织已停止生长，而４个变异系愈伤组织能较正常生长。在无盐胁迫下，变异系脯氨酸和可溶性蛋白含量均显著高于对照系，细胞膜透性显著低于对照系。在不同浓度ＮａＣｌ胁迫下，４个变异系和对照系的脯氨酸含量、可溶性蛋白含量及细胞膜透性随ＮａＣｌ浓度的增加而增加，但４个变异系增加幅度较缓，对照系增加幅度较大。在相同ＮａＣｌ浓度下，４个变异系的脯氨酸含量、可溶性蛋白含量仍显著高于对照系，细胞膜透性显著低于对照系。４个变异系对ＮａＣｌ胁迫的耐性较强
With the accumulation of four proline variants in the same-genotype grape, the salt tolerance of proline accumulation in grape was studied under different NaCl stress. The results showed that the accumulation of proline in grape was strongly resistant to high NaCl stress. Under the stress of 240 mmol·L-1, the callus of the control line had stopped growing, while the four callus lines could grow more than normal. Under salt stress, the contents of proline and soluble protein in the mutant lines were significantly higher than those in the control lines, and the membrane permeability was significantly lower than that in the control lines. The proline content, soluble protein content and cell membrane permeability of the four mutant lines and the control lines increased with the increase of NaCl concentration under the different concentrations of NaCl stress, but the four mutation lines increased more slowly than the control lines Big. Under the same NaCl concentration, the proline content and soluble protein content of the four mutant lines were still significantly higher than that of the control line, and the membrane permeability of the four mutant lines was significantly lower than that of the control line. Four mutant lines were more resistant to NaCl stress