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到目前为止检测IL-2的首选方法仍然是通过IL-2在体外促进T细胞的生长来定量其活性.虽然这一方法极为敏感以至可测出200pg/ml的IL-2含量,但它对许多干扰因素也很敏感,如细胞毒药物可掩盖IL-2的活性,其它物质如PMA(Phorbol Myristic Acetatc)也效仿或协同IL-2作用于某些靶细胞.检测临床标本中的IL-2时更因正常人血清中一些抑制物质的存在而变得复杂化.针对上述问题,作者设计了一种ELISA方法,它可快速、不受一些抑制物质影响地定量检测IL-2.
The preferred method to detect IL-2 so far is still to quantify its activity by promoting the growth of T cells in vitro by IL-2.Although this method is so sensitive that an IL-2 level of 200 pg / ml can be measured, Many interfering factors are also sensitive, such as cytotoxic drugs can mask the activity of IL-2, other substances such as PMA (Phorbol Myristic Acetatc) also follow the IL-2 or synergistically act on certain target cells.Detection of IL-2 But also more complicated by the presence of some inhibitory substances in normal human serum.In response to the above problems, the authors designed an ELISA method that allows for the rapid quantitation of IL-2 without the influence of some inhibitory substances.