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①目的建立定量聚合酶链反应（ＰＣＲ）方法，检测宫颈组织中人乳头瘤病毒１６型（ＨＰＶ１６）的基因含量。②方法通过重叠延伸ＰＣＲ构建一含ＥｃｏＲＩ酶切位点的内参照模板，用竞争性ＰＣＲ（ＣＰＣＲ）检测６例ＨＰＶ１６阳性宫颈非典型增生组织标本中ＨＰＶ１６Ｅ６基因的拷贝数。③结果６例阳性标本ＨＰＶ１６Ｅ６基因的拷贝数为每毫克ＤＮＡ中含１．９９×１０５～２．５０×１０７．④结论用ＣＰＣＲ检测宫颈组织中ＨＰＶ１６Ｅ６基因的含量是可行的，进一步比较不同宫颈疾患组织中ＨＰＶ１６Ｅ６基因的含量，可以从分子水平探讨ＨＰＶ１６的致癌机理。
Objective To establish a quantitative polymerase chain reaction (PCR) method to detect the gene expression of human papillomavirus type 16 (HPV16) in cervical tissue. Methods The internal reference template containing EcoRI restriction sites was constructed by overlap extension PCR. The copy number of HPV16E6 gene in 6 HPV16-positive cervical atypical hyperplasia tissues was detected by competitive PCR (CPCR). Results The copy number of HPV16E6 gene in 6 positive samples was 1.99 × 105 ~ 2.50 × 107 per mg of DNA. ④Conclusion It is feasible to detect the content of HPV16E6 gene in cervical tissue by CPCR. To further compare the content of HPV16E6 gene in different cervical tissues, we can explore the carcinogenic mechanism of HPV16 from the molecular level.