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本文比较和分析了抗旱性不同的甘蔗品种的愈伤组织对高渗溶液(渗透剂为聚乙二醇PEG6000)的生理反应差异。结果表明,在正常条件下,抗旱性不同的品种其愈伤组织在TTC还原力、游离脯氨酸含量、可溶性蛋白质含量、过氧化物酶活性、膜相对透性和极性膜脂配比上均无显著差异。经30％PEG高渗溶液(—12.0巴)处理24小时后,抗旱型的愈伤组织相对还原力和游离脯氨酸含量明显高于不抗旱型的;并且愈伤组织饱和脂肪酸含量增加较多,而不抗旱型的增加较少;可溶性蛋白质含量、过氧化物酶活性和膜相对透性在抗旱型与不抗旱型的愈伤组织之间差异不显著。作者认为,大田抗旱甘蔗品种具有细胞学基础,经水分胁迫处理后,愈伤组织相对TTC还原力和游离脯氨酸含量可能成为甘蔗抗旱指标。
In this paper, we compared and analyzed the physiological responses of the callus of the different drought resistance sugarcane varieties to hypertonic solution (penetrant polyethylene glycol PEG6000). The results showed that under normal conditions, the callus of different callus cultivars with different drought resistance had significant differences in TTC reducing force, free proline content, soluble protein content, peroxidase activity, membrane relative permeability and polar membrane lipid ratio No significant difference. After treated with 30% PEG hypertonic solution (-12.0 bar) for 24 hours, the relative reducing power and free proline content of drought-resistant callus were significantly higher than those of non-drought-tolerant type; and the content of saturated fatty acids in callus increased more , But no increase in drought-resistant type. There was no significant difference in soluble protein content, peroxidase activity and membrane relative permeability between drought-resistant and non-drought-resistant callus. The authors believe that Datian drought-resistant sugarcane varieties have cytological basis, after water stress treatment, callus relative TTC reducing power and free proline content may be sugarcane drought resistance index.