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目的探讨HLA-C基因5’端-35kb C/T多态性(Rs9264942)在四川汉族HIV感染中的影响。方法采用序列特异性引物扩增(PCR-SSP)方法对四川汉族259例HIV感染进展组,46例HIV感染长期不进展组,和270例四川汉族对照组人群HLA-C基因5’UTR区域-35kb C/T的多态性进行分型。结果 -35kb C/T多态性在HIV感染进展组,HIV感染不进展组及正常对照组人群中各等位基因分布符合Hardy-Weinberg平衡,在HIV感染进展组中:CC频率为15.1%、CT频率41.3%、TT频率43.6%;HIV感染不进展组中:CC频率为21.7%、CT频率39.1%、TT频率39.1%;在正常对照组中:CC频率为18.9%、CT频率43.0%、TT频率38.1%。经统计学检验,-35kb C/T多态性中各等位基因频率在3组人群中没有统计学差异。结论 HLA-C基因-35kb C/T多态性与四川汉族HIV感染者的感染进程没有相关性。
Objective To investigate the effect of Rs9264942 on the HIV infection in Han nationality in Sichuan province. Methods The sequence of PCR-SSP was used to analyze the prevalence of HLA-C gene 5’-UTR in 259 cases of progression of HIV infection, 46 cases of long-term non-HIV-infected progression in Sichuan Han population, 270 cases of Sichuan Han control group, 35kb C / T polymorphism for typing. Results The -35kb C / T polymorphism was consistent with Hardy-Weinberg equilibrium in HIV-infected progression group, HIV-infected non-progression group and normal control group. In HIV-infected progression group, the CC frequency was 15.1% The frequency of CT was 41.3% and the frequency of TT was 43.6%. In HIV-infected patients, the CC frequency was 21.7%, the CT frequency was 39.1% and the TT frequency was 39.1%. In the normal control group, the CC frequency was 18.9%, the CT frequency was 43.0% TT frequency of 38.1%. Statistically, the -35kb C / T polymorphism in the allele frequency in the three groups of people no statistically significant difference. Conclusion The -35kb C / T polymorphism of HLA-C gene has no correlation with the infection progress of HIV-infected Han in Sichuan province.