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近年来各地开展链球菌溶血素“O”的制备。普遍采用过去有关资料介绍的方法:将溶血性链球菌接种于10毫升肉汤培养基内,培养8小时,以1:100的比例培养液移入大瓶培养基,培养16～18小时或有的培养24小时后,就收集溶血素“O”。我们通过实践初步掌握了链球菌在培养过程中几个关键环节,并对产生溶血素“O”的影响因素的控制,使培养时间减少一半,又能制出高效价的溶血素“O”。现将有关制作方法介绍如下。
In recent years, streptolysin “O” has been prepared all over the world. Commonly used in the past, the method described in the relevant information: Streptococcus hemolyticus inoculated in 10 ml of broth medium, cultured for 8 hours, the ratio of 1: 100 culture medium into large bottle medium, cultured for 16 to 18 hours or some culture After 24 hours, hemolysin “O” was collected. In practice, we have mastered several key aspects of streptococcal culture, and controlled the factors that influence the production of hemolysin “O”, reducing the culturing time by half and producing high titer hemolysin “O”. The production methods are introduced below.