论文部分内容阅读
耳蜗内静息电位产生的机理尚不清楚。该文目的是通过检测在正常状态及缺氧状态下边缘细胞的静息电位及单价离子浓度,来解释静息电位产生的原理。实验采用健康豚鼠,去除一小块蜗管骨壁,暴露基底转的血管纹,将电极插入血管纹不同部位,同时将一离子敏感微电极通过螺旋韧带插入蜗管。结果缺氧20分钟后,边缘细胞的静息电位由62.7mV降至-2.4mV,K~+活性由77.7mEq/L降至53.2mEq/L,而Na~+活性由2.6mEq/L升至24.7mEq/L,CI-活性变化不明显。在蜗管内,静息电位在缺氧
The mechanism of resting potentials in the cochlea is unclear. The purpose of this paper is to explain the principle of resting potential generation by detecting the resting potential and monovalent ion concentration of marginal cells in normal and hypoxic conditions. In this experiment, healthy guinea pigs were used to remove a small cochlear wall and expose the basal veins. The electrodes were inserted into different parts of the vascular pattern, and an ion-sensitive microelectrode was inserted into the cochlear duct through the spiral ligament. Results After 20 minutes of hypoxia, resting potential of peripheral cells decreased from 62.7mV to -2.4mV, K ~ + activity decreased from 77.7mEq / L to 53.2mEq / L, and Na ~ + activity increased from 2.6mEq / L to 24.7mEq / L, CI-activity did not change significantly. In the scroll, resting potential in hypoxia