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目的探讨出生前邻苯二甲酸二(2-乙基己基)酯(DEHP)暴露对大鼠肾上腺功能的潜在干扰作用。方法将32只健康SD孕鼠按体重随机分为溶剂对照组(玉米油)及2、10、50 mg/kg DEHP染毒组,每组8只。自孕14~19 d连续灌胃染毒,每日1次。子代大鼠于出生后第21天断乳,雌雄分笼饲养至70日龄,断头处死,取血及双侧肾上腺,计算肾上腺脏器系数,并采用Luminex液态芯片技术测定血清皮质醇水平。结果成年雌性仔鼠肾上腺脏器系数在各染毒组间有差异,与对照组相比,2 mg/kg染毒组的肾上腺重量及脏器系数较低,差异有统计学意义(P<0.05);成年雄性仔鼠肾上腺脏器系数在各染毒组间差异均无统计学意义(P>0.05)。与对照组相比,10、50 mg/kg染毒组的成年雌性仔鼠血清皮质醇水平较高,差异有统计学意义(P<0.01);而2 mg/kg染毒组与对照组相比无明显差异(P>0.05)。与对照组相比,10 mg/kg染毒组的成年雄性仔鼠血清皮质醇水平较高,差异有统计学意义(P<0.01);而2、50 mg/kg染毒组与对照组相比无明显差异(P>0.05)。结论出生前DEHP暴露可能干扰子代大鼠肾上腺功能。
Objective To investigate the potential interference of prenatal exposure to di-2-ethylhexyl phthalate (DEHP) on adrenal function in rats. Methods 32 healthy pregnant SD rats were randomly divided into solvent control group (corn oil) and 2, 10, 50 mg / kg DEHP exposure group, with 8 rats in each group. From 14 to 19 d of pregnancy by intragastric administration of poisoning, 1 day. The offspring rats were weaned on the 21st day after birth, the male and female were kept in the cage for 70 days, sacrificed, the blood and bilateral adrenal were collected, the coefficient of the adrenal organ was calculated, and the level of serum cortisol was measured by Luminex liquid chip technique . Results The coefficient of adrenal glands in adult female offspring rats was different among the groups. Compared with the control group, the adrenal weight and organ coefficient of 2 mg / kg group were lower (P <0.05 ). There was no significant difference in the coefficient of adrenal organ in adult male offspring between the two exposure groups (P> 0.05). Compared with the control group, serum cortisol levels in adult female offspring of 10,50 mg / kg group were significantly higher (P <0.01), while those in 2 mg / kg group No significant difference (P> 0.05). Compared with the control group, serum cortisol levels in adult male offspring of 10 mg / kg exposure group were significantly higher (P <0.01), while those of 2 and 50 mg / kg exposure groups No significant difference (P> 0.05). Conclusion Prenatal DEHP exposure may interfere with adrenal function in offspring rats.