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本研究旨在探索一种便于大规模筛选小麦种质的方法。这种方法是以叶组织中ABA含量及其积累速率为选择标准的。利用直接酶免疫法研究了提取时间、C_(18)反向色层、溶剂挥发以及样本提取液的稀释对ABA定量的影响。结果表明,提取溶剂的完全挥发不影响ABA测定。利用800ml/L甲醇从小麦叶片提取ABA需用24h。甲醇稀释浓度≤80ml/L时,消除了由甲醇造成的对酶免疫法的干扰。稀释曲线的对应性测试或利用C_(18)反向色层分离法对叶提取液的部分纯化都未检测到对酶免疫法的明显抑制因子。
The purpose of this study was to explore a method for large-scale screening of wheat germplasm. This method is based on ABA content in leaf tissue and its rate of accumulation as the selection criteria. The effects of extraction time, C 18 reverse chromatography, solvent evaporation and dilution of sample extract on ABA quantification were studied by direct enzyme immunoassay. The results show that the complete volatilization of the extraction solvent does not affect the ABA assay. The extraction of ABA from wheat leaves with 800ml / L methanol takes 24h. Methanol dilution of ≤ 80ml / L, eliminating the interference caused by methanol on the enzyme immunoassay. Dilution curve of the corresponding test or the use of C_ (18) reverse chromatographic separation of leaf extract were not detected for the partial purification of significant inhibitory factor for enzyme immunoassay.