用含有腺病毒EIA和EIB基因的DNA片段对一株由猴病毒40(SV_(40))转化的大鼠肝细胞系——CWSV-1细胞进行了转化实验,以了解腺病毒基因和SV_(40)DNA之间的关系。我们的结论是:(1)腺病毒DNA能转化CWSV-1细胞;(2)建立的NP细胞呈重叠生长趋势,当细胞复盖培养基达60％左右时,大部份细胞自行脱落,但细胞形态没有明显改变;(3)形成的NP细胞系大部份不再分泌可检测的白蛋白,少量细胞分泌白蛋白的能力也随着传代而消失。 A rat liver cell line, CWSV-1 transformed with simian virus 40 (SV_ (40)), was transformed with a DNA fragment containing adenovirus EIA and EIB genes to understand the relationship between the adenovirus gene and SV_ ( 40) The relationship between DNA. We conclude that: (1) adenovirus DNA can transform CWSV-1 cells; (2) the established NP cells showed an overlapping growth trend. When the cells covered 60% of the culture medium, most of the cells shed themselves, Cell morphology did not change significantly; (3) most of the formed NP cell lines no longer secrete detectable albumin, the ability of a small number of cells to secrete albumin disappeared with passage.