用荧光探针法(以Fura-2为荧光指示剂)通过监测细胞内荧光强度的变化来判断稀土的跨膜行为.在近生理条件下,从单细胞、多细胞两个角度,研究了人外周血淋巴细胞钙离子通道开放、关闭等不同状态Dy~(3+),La~(3+)等稀土离子的跨膜行为.实验得出:微量稀土离子在上述状态下均不能跨膜内流.我们还发现,在钙通道开放的情况下,低剂量稀土离子不影响细胞外钙跨膜内流. The transmembrane activity of rare earths was determined by monitoring the change of intracellular fluorescence intensity by fluorescent probe method (using Fura-2 as fluorescence indicator). Under near physiological conditions, from the perspective of single cell and multicellular, The transmembrane behaviors of rare earth ions, such as Dy 3+ and La 3+, in different states such as opening and closing of calcium channels in peripheral blood lymphocytes were studied.The results show that all trace rare earth ions can not transmembrane in the above state We also found that low doses of rare earth ions do not affect extracellular calcium transmembrane influx in the presence of calcium channels.