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为了解根内生细菌群落结构在土壤侵蚀条件下的变化,明确有机肥施用对其多样性的影响,本研究采用LNA-PCR和454高通量测序技术相结合的方法对土壤表层剥离30 cm和施用有机肥处理的玉米苗期根内生细菌群落结构进行研究,以表层土没有剥离和单施化肥处理为对照.试验共获得37820条16S r DNA有效序列,主要分布在4个门35个纲214个属和782个OTU,其中,变形菌门、厚壁菌门、放线菌门和拟杆菌门为优势菌门,但不同样品各菌门分布比例存在差异.土壤侵蚀降低了玉米苗期根内生细菌群落多样性,但增施有机肥可以增加根内生细菌群落多样性,这种作用以表层土剥离30 cm处理表现得更为突出.
In order to understand the changes of endophytic bacterial community structure under soil erosion conditions and to clarify the effects of organic manure application on its diversity, we used LNA-PCR and 454 high-throughput sequencing to separate 30 cm And organic manure treatment of corn seedling root endophytic bacterial community structure to study the topsoil without stripping and fertilizer alone as a control test were obtained 37820 16S rDNA valid sequences are mainly distributed in four gates 35 214 genera and 782 OTUs, of which, Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were the dominant fungi, but there were differences in the proportion of each fungi in different samples. Soil erosion reduced the content of maize seedlings Endophytic bacterial community diversity, but the organic manure could increase the diversity of endophytic bacterial community, which was more prominent when the topsoil stripped 30 cm.