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目的:探讨宝肾方对糖尿病大鼠的治疗作用及其对肾组织结缔组织生长因子(CTGF)和基质金属蛋白酶-9(MMP-9)表达的影响。方法:以链脲佐菌素(STZ)诱导建立糖尿病大鼠模型。随机分为对照组(C组)、糖尿病组(D组)和糖尿病宝肾方治疗组(DB组)。给药8周后检测各组生化指标,观察肾组织病理形态学改变,采用免疫组化方法分析肾组织CT-GF和MMP-9蛋白的表达情况。结果:和对照组相比,糖尿病组肾组织CTGF蛋白表达明显升高[(0.234±0.009)vs(0.146±0.007),P(0.01],而MMP-9蛋白表达明显降低[(0.146±0.006)vs(0.236±0.007),P(0.01],肾脏肥大指数、24 h尿蛋白、血糖、胆固醇、血肌酐明显升高(P(0.01);和糖尿病组相比,宝肾方治疗组肾组织CTGF蛋白表达降低[(0.194±0.008)vs(0.234±0.009),P(0.01],而MMP-9蛋白表达升高[(0.175±0.007)vs(0.146±0.006),P(0.01],肾脏肥大指数降低(P(0.05),24 h尿蛋白、胆固醇、血肌酐降低(P(0.01)。结论:宝肾方对糖尿病大鼠肾脏具有保护作用,其机制可能与影响肾组织CTGF和MMP-9的表达有关。
Objective: To investigate the therapeutic effect of Baoshen Prescription on diabetic rats and its effect on the expression of connective tissue growth factor (CTGF) and matrix metalloproteinase-9 (MMP-9) in renal tissue. METHODS: A diabetic rat model was induced by streptozotocin (STZ). They were randomly divided into control group (C group), diabetes group (D group) and diabetic Baoshenfang treatment group (DB group). After 8 weeks of administration, the biochemical indicators of each group were examined to observe the pathological changes of renal tissues. The expression of CT-GF and MMP-9 protein in renal tissues was analyzed by immunohistochemistry. Results:Compared with the control group, the expression of CTGF protein in the kidney of diabetic group was significantly increased [(0.234±0.009) vs (0.146±0.007), P(0.01), but the expression of MMP-9 protein was significantly decreased [(0.146±0.006) Vs (0.236±0.007), P(0.01), Renal hypertrophy index, 24 h urinary protein, blood glucose, cholesterol, and serum creatinine were significantly increased (P(0.01); Compared with diabetic group, kidney tissue CTGF in Baoshenfang treatment group. Protein expression decreased [(0.194±0.008) vs (0.234±0.009), P (0.01), and MMP-9 protein expression increased [(0.175±0.007) vs (0.146±0.006), P(0.01), renal hypertrophy index Decreased (P(0.05), 24 h urinary protein, cholesterol, serum creatinine decreased (P(0.01). Conclusion: Baoshen Prescription has protective effect on kidney of diabetic rats, and its mechanism may be related to the influence of renal tissue CTGF and MMP-9 Expression related.