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许多研究报告表明,低密度脂蛋白(LDL)可经多种途径被氧化修饰成ο-LDL,巨噬细胞对ο-LDL的降解速率较LDL快3～10倍,动脉粥样硬化损伤部位的泡沫细胞是由巨噬细胞聚集了大量的胆固醇而形成的,LDL的氧化修饰以及由此导致的巨噬细胞吞噬的增加,对泡沫细胞的形成起到重要作用,本项研究采用紫外线照射和Cu~(2+)-PBS系统分别对LDL进行氧化修饰并且观察了绿茶多酚(10μg·ml~(-1))和黄酮类化合物桑色素(8.5μg·ml~(-1))对这一化学过程的抑制作用,其结果表明,两者均对LDL的氧化修饰具有显著抑制作用,主要实验证据为,它们可使琼脂糖电泳迁移率,硫代巴比妥酸反应物和激发光为360 nm,发射光为420 nm时的荧光强度等的增加量抑制50％左右并使小鼠腹腔巨噬细胞降解o-LDL的速率抑制70％,绿茶多酚和桑色素分别存在于绿茶和蔬菜中,因此它们防止LDL的氧化修饰对于动脉粥样硬化的预防和治疗具有一定的临床意义。
Many studies have shown that low density lipoprotein (LDL) can be oxidized to ο-LDL by a variety of ways, macrophages on o-LDL degradation rate of 3 to 10 times faster than LDL, atherosclerotic lesion site Foam cells are formed by macrophages that accumulate large amounts of cholesterol. The oxidative modification of LDL and the increase of macrophage phagocytosis play an important role in the formation of foam cells. In this study, UV irradiation and Cu LDL was oxidatively modified by ~ (2 +) - PBS system and the effects of green tea polyphenols (10μg · ml -1) and flavonoid morin (8.5μg · ml -1) The results showed that both of them have significant inhibitory effect on the oxidative modification of LDL. The main experimental evidence is that they can make agarose electrophoresis mobility, thiobarbituric acid reactants and excitation light 360 nm, the fluorescence intensity at 420 nm emission was inhibited by about 50% and the rate of o-LDL degradation by peritoneal macrophages was inhibited by 70%. Green tea polyphenols and morin were present in green tea and vegetables, respectively , They prevent the oxidative modification of LDL against atherosclerosis Prevention and treatment of certain clinical significance.