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目前认为,某些肿瘤的生长和转移,是由于宿主体内存在的免疫抑制细胞(Suppressor cells)抑制并取消抗肿瘤的免疫反应所致。免疫抑制细胞的一个特点是在其表面存在H_2型组胺受体,并易被组胺所激活。特异性H_2受体拮抗剂甲氰咪胍(Cimetidine)则可阻滞组胺的激活并减弱免疫抑制细胞的功能。在Lewis肺癌(3LL)小鼠研究甲氰咪胍对免疫抑制细胞功能、肿瘤的转移以及对宿主存活的影响,结果发现,甲氰咪胍能明显抑制免疫抑制细胞的功能。作者测定经组胺激活后的离体脾淋巴细胞对植物血凝素(PHA)的效应,以检测免疫抑制细胞的功能活性。结果对照组由组胺激活后的抑制百分率为48±6%;甲氰咪胍组为21±4%(p<0.05)。甲氰咪胍还能明显减少肿瘤转移并显著延长小鼠存活期。给C57BL/6小鼠的右后肢足跖接种3LL肿瘤细胞,当局部肿瘤直径到达7.5mm时(一般于注射后7～9天),对足及腘邻近淋巴结
It is currently believed that the growth and metastasis of certain tumors is due to suppression and elimination of anti-tumor immune responses by Suppressor cells present in the host. One of the hallmarks of immunosuppressed cells is the presence of H 2 -type histamine receptors on their surface and their susceptibility to histamine activation. Cimetidine, a specific H 2 receptor antagonist, blocks histamine activation and attenuates immunosuppressive cell function. The effects of cimetidine on immunosuppressive cell function, tumor metastasis, and host survival were studied in Lewis lung carcinoma (3LL) mice and found that cimetidine significantly inhibited the function of immunosuppressive cells. The authors determined the effect of histamine-activated ex vivo splenic lymphocytes on phytohemagglutinin (PHA) to test the functional activity of immunosuppressed cells. Results The percent inhibition after histamine activation was 48 ± 6% in the control group and 21 ± 4% in the cimetidine group (p <0.05). Cimetidine also significantly reduced tumor metastasis and significantly prolonged mouse survival. 3LL tumor cells were inoculated into the right hind limbs of C57BL / 6 mice. When the local tumor diameter reached 7.5mm (usually 7-9 days after injection)