论文部分内容阅读
研究目的:β-淀粉酶活性(BAA)与热稳定性(BAT)是啤用大麦品质的重要性状,本研究旨在明确青藏高原一年生野生大麦BAA和BAT的基因型差异,鉴定可用于啤用大麦品质育种的特异种质材料。创新要点:青藏高原一年生野生大麦中具有高BAA和BAT的基因型,可为啤用大麦品质育种提供特异遗传材料;解析了不同温度与高温处理不同时间对BAT的影响及其机理。研究方法:本研究以138个青藏高原一年生野生大麦基因型和20个栽培大麦品种为材料,在杭州大田条件下种植两年(2008~2009年和2011~2012年),利用Megazyme公司研制的试剂盒BETAMYL-3?kit法测定BAA,用两点法测定BAT。重要结论:BAA和BAT在供试的基因型之间均有显著的差异,且野生大麦的基因型变异明显大于栽培品种,青藏高原一年生野生大麦中具有高BAA和BAT值的野生大麦材料。
Research purposes: β-amylase activity (BAA) and thermal stability (BAT) are important traits of barley quality for beer. The aim of this study was to determine the genotype difference of BAA and BAT in annual wild barley from Qinghai-Tibet Plateau. Barley quality breeding of specific germplasm material. Innovative Points: Genotypes with high BAA and BAT among annual wild barley in the Qinghai-Tibet Plateau can provide specific genetic material for barley quality breeding. The effects of different temperature and high temperature treatments on BAT and its mechanism were analyzed. Research methods: In this study, 138 annual wild barley genotypes from Qinghai-Tibet Plateau and 20 cultivated barley cultivars were planted in Hangzhou under field conditions for two years (2008 ~ 2009 and 2011 ~ 2012), using reagents developed by Megazyme BAAM was measured by BETAMYL-3 kit method and BAT was determined by two-point method. Important conclusions: There was a significant difference between BAA and BAT genotypes tested, and the genotypic variation of wild barley was significantly greater than that of cultivated species. Wild barley material with high BAA and BAT in annual wild barley from the Qinghai-Tibetan Plateau was significantly higher.