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〔日〕/古谷美野里…探讨了伞形科多年生草本植物阿魏的提取成分诱导细胞凋亡及对MBP激酶的作用。方法:①Jurkat细胞及HL-60(clone15)细胞以含10%FBS及2mM L-谷氨酰胺的培养液常规培养。用PI/Annexin V-FITC染色后,以流式细胞仪检测细胞凋亡。②以In-gel激酶及蛋白质印迹法观察
〔〕 〕 / Furuya Miyoshiri ... explored the Umbelliferous perennial herb Ferula extract components induced apoptosis and the role of MBP kinase. Methods: 1 Jurkat cells and HL-60 (clone 15) cells were routinely cultured in a medium containing 10% FBS and 2 mM L-glutamine. After staining with PI/Annexin V-FITC, apoptosis was detected by flow cytometry. 2 observed by In-gel kinase and Western blot