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目的:观察白假丝酵母菌标准菌株(CCCMC1a、ATCC 14053)与临床分离菌株(C1-1、C1-2、C1-3、C1-4)于不同条件下荚膜层的厚度变化。方法:(1)将实验菌种沙保氏琼脂36小时培养物用生理盐水配成约含1×109个/ml的菌悬液,家兔耳缘静脉注射,1ml/只,同时用BALB/c小鼠腹腔注射,0.5ml/只;待实验动物发病后解剖动物,分别取家兔肾组织印片及小鼠腹腔液涂片进行Hiss荚膜染色镜检,用显微测微计测量荚膜层的厚度,各菌株随机计数40个酵母细胞的荚膜层的厚度值,取各菌株荚膜层厚度的均值进行比较。(2)实验菌株沙保氏液体培养物与同一菌株的抗血清作荚膜肿胀实验及血清交叉实验,Hiss荚膜染色镜检。荚膜层厚度的测量与计算方法同前。结果:(1)标准菌株与临床分离菌株在家兔及小白鼠体内均可形成荚膜,荚膜层的厚度可因不同菌种(株)和不同环境而有差异(均值在0.25~0.58μm不等;P<0.01或<0.05),边界明显。(2)用全菌抗血清作荚膜肿胀实验,可见菌细胞的荚膜明显加宽,且菌株之间可出现交叉阳性。结论:白假丝酵母菌的荚膜厚度可因不同菌种(株)和不同环境而有差异;荚膜肿胀实验显示各菌株除与相应抗血清作用出现荚膜肿胀实验阳性外菌株之间还可出现血清交叉阳性。
Objective: To observe the change of capsule thickness under different conditions of Candida albicans standard strain (CCCMC1a, ATCC 14053) and clinical isolates (C1-1, C1-2, C1-3, C1-4). Methods: (1) The experimental bacteria Sabouraud agar 36 hours culture with physiological saline dubbed containing about 1 × 109 / ml bacterial suspension, rabbits ear vein injection, 1ml / only, while BALB / c mice were injected intraperitoneally, 0.5ml / only; to be dissected animals after the onset of experimental animals, respectively, take rabbit kidney tissue print and mouse peritoneal fluid smear Hiss capsule microscopy, using a micrometer to measure pods The thickness of the membrane layer, each strain randomly counted the thickness of the capsular layer of 40 yeast cells, and took the average of the capsule layer thickness of each strain for comparison. (2) Experimental strains of Sabouraud liquid culture with the same strain of antiserum for capsular swelling test and serum cross test, Hiss capsule staining. Capsule thickness measurement and calculation methods with the previous. Results: (1) The standard strains and clinical isolates could form capsular in rabbits and mice, and the thickness of the capsular layer could be different due to different strains (strains) and different environments (mean 0.25 ~ 0.58μm Range; P <0.01 or <0.05), the border is obvious. (2) With whole-cell antiserum for capsular swelling test, we can see that the capsule of bacterial cells is obviously broadened, and cross-positive can occur between the strains. Conclusion: The capsular thickness of Candida albicans can be varied according to different strains (strains) and different environments; capsular swelling test showed that in addition to the role of the corresponding antiserum capsular swelling test was positive between the strains Seropositive cross-positive can occur.