论文部分内容阅读
作者应用免疫细胞化学法研究了HFRS尸检内分泌及其有关组织中病毒抗原的分布,病毒抗原阳性细胞与病变的关系,病变的类型及病毒感染对胰腺内胰岛素表达的影响,所用的一抗有抗肾综合征出血热(HFRS)病毒糖蛋白(G_2),核蛋白(NP)及血凝素(HA)抗原的单克隆抗体(15种)和多克隆抗体(PAb)以及抗胰岛素(INS)单克隆抗体。结果显示,内分泌组织中有两种染色形态的病毒抗原,一种是胞浆弥漫染色的抗原,为G_2,NP或HA阳性,阳性细胞弥漫分布,另一种是以病毒包涵体(IB)形式出现的颗粒性抗原,MAbs染色多为实性,PAb染色多为空泡状。在9例胰腺组织中,有4例几乎在所有腺上皮细胞和少数胰岛细胞均为IB阳性,但PAb染色可在胰岛细胞中显示出空泡状IB。在其它内分泌组织中,IB只在少数细胞中出现。抗原阳性细胞很少出现坏死,但在脑垂体及肾上腺灶性或片状凝固性坏死区域也有弥漫性抗原阳性及IB存在。胰腺组织的胰岛素染色显示,除1例胰岛INS强阳性,其余8例均有明显的INS染色减弱及阳性细胞减少的表现。结果提示,内分泌组织中有两种病变,即以IB为特征的病毒直接感染引起的细胞病变,是病毒的弱致病变作用,该病变可能对细胞的内分泌功能产生影响;另一种病变是组织的灶性或大片性同步性凝固性坏死,是继发于病毒感染后的出血和休克,该病变会
The authors used immunocytochemistry to study the distribution of viral antigens in endocrine and related tissues in HFRS autopsy, the relationship between viral antigen-positive cells and lesions, the type of lesions and the effect of virus infection on the expression of insulin in the pancreas. The primary anti- Monoclonal antibodies (15) and polyclonal antibodies (PAb) and anti-insulin (INS) antibodies against HFRS virus glycoprotein (G_2), nucleoprotein (NP) and hemagglutinin (HA) Clone antibody. The results showed that there are two kinds of endocrine staining of the virus antigen, one is the cytoplasm diffuse staining antigen, G_2, NP or HA positive, diffuse distribution of positive cells, and the other is in the form of viral inclusions (IB) There are granular antigens, MAbs stain mostly solid, PAb mostly vacuolar. Of the 9 cases of pancreatic tissue, 4 showed IB positive in almost all glandular epithelial cells and a few islet cells, but PAb staining showed vacuolar IB in islet cells. In other endocrine tissues, IB appears only in a few cells. Antigen positive cells rarely necrosis, but in the pituitary and adrenal focal or focal coagulation necrosis area also has diffuse antigen-positive and IB exist. Insulin staining of pancreatic tissue showed that in all but one of the islets, there was a significant decrease of INS staining and positive cells in all but 8 cases. The results suggest that there are two kinds of pathological changes in the endocrine tissue: the cytopathic effect caused by the direct infection of the virus characterized by IB is the weak pathogenic effect of the virus, which may have an impact on the endocrine function of the cell; the other is Focal or large syncytial tissue coagulation necrosis is secondary to post-viral infection bleeding and shock, the lesion will be