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前期的研究已经证实了转铁蛋白(TRF)能抑制FSH诱导的抑制素、雌二醇和孕酮的产生以及芳香化酶活性。本实验用已烯雌酚处理后分离的大鼠卵泡颗粒细胞的体外培养方法,进一步观察TRF对FSH、霍乱毒素(cholera toxin,CT)及Forskolin诱导的cAMP、雌二醇及孕酮产生的影响。结果:TRF抑制了FSH诱导的cAMP、雌二醇及孕酮产生TRF能抑制CT及Forskolin诱导的雌二醇产生,但不影响这些因子刺激cAMP和孕酮生成。说明TRF抑制FSH对大鼠卵泡颗粒细胞功能性分化刺激作用的主要细胞内机制是通过减少cAMP生成而介导的,但还有cAMP以外的作用部位。
Preliminary studies have demonstrated that transferrin (TRF) inhibits FSH-induced production of statins, estradiol and progesterone, and aromatase activity. In this study, diethylstilbestrol treated rat granulosa cells isolated in vitro methods to further observe the TRF FSH, cholera toxin (cholera toxin (CT) and Forskolin-induced cAMP, estradiol and progesterone production . RESULTS: TRF inhibited FSH-induced cAMP, estradiol and progesterone-induced TRF inhibited CT and Forskolin-induced estradiol production, but did not affect these factors to stimulate cAMP and progesterone production. The main intracellular mechanism that TRF inhibits the stimulatory effect of FSH on the functional differentiation of granulosa cells in rats is mediated through the reduction of cAMP production but there are other sites of action other than cAMP.