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在精子发生后期,精子细胞核内与DNA结合的体细胞类型组蛋白逐步地被鱼精蛋白替代,使精子细胞核变长,高度浓缩,最终分化成为成熟的精子,鱼精蛋白是成熟精子细胞核内与DNA结合的主要蛋白质.从鱼类精子分离纯化的鱼精蛋白对培养的人宫颈癌细胞HeLa有显著抑制作用.其IC_(50)为50μg/ml.经鱼精蛋白处理过的HeLa细胞生长速度明显减慢,在软琼脂中丧失形成集落的能力,~3H-TdR的参入率也明显降低,当浓度为40μg/ml时,参入率仅为对照的50％,当浓度达到120μg/ml时,参入率接近零。
In the late stage of spermatogenesis, the somatic cell type histone bound to DNA in the sperm nucleus is gradually replaced by protamine to make the sperm nucleus become longer, highly concentrated and eventually differentiated into mature spermatozoa, which is the nucleus of mature spermatid DNA-bound major protein.The purified protamine from fish sperm had a significant inhibitory effect on HeLa cultured human cervical cancer cells.Its IC 50 was 50μg / ml.The protamine-treated HeLa cells grew rapidly Significantly slowed down the ability to form colonies in soft agar, ~ 3H-TdR incorporation rate was significantly reduced when the concentration of 40μg / ml, the participation rate was only 50% of the control, when the concentration reached 120μg / ml, Participation rate close to zero.