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对有关液体中银或者银离子除菌机理的研究进行了报道,使用近紫外光线( 395nm)在光照射以及非光照射条件下对溶液中银离子的除菌作用进行了检测对比。对光照射所引起的银离子光催化反应以及伴随其产生的对微生物的抑制增殖作用进行了探讨。实验中以金黄色葡萄球菌作为原核微生物的代表,以白色念珠菌和须癣毛癣菌作为真核微生物的代表,检测并对比了光照射条件下银离子溶液对原核微生物和真核微生物的抑制增殖作用。实验结果显示,在光照射下溶液中银离子对这两类微生物的抑制增殖作用大大高于无光照银离子溶液的作用。通过使用自由基捕获剂,利用电子自旋共振法,检测到由银离子光催化反应产生的大量羟自由基(·OH),即光照射下溶液中产生活性氧基(·OH)。由此可以推想在光照射下溶液中活性氧基(·OH)和银离子同时攻击微生物细胞,破坏细胞壁(或细胞膜)以及细胞内酶,基因等,从而使溶液显示强大的除菌能力。
Studies on the mechanism of sterilizing silver or silver ions in liquid have been reported. Near-UV light (395nm) was used to detect and compare the bactericidal effect of silver ions in solution under light and non-light irradiation conditions. The photocatalytic reaction of silver ions caused by light irradiation and the inhibitory and proliferative effect on the microorganisms accompanying with it were also discussed. In the experiment, Staphylococcus aureus was taken as the representative of prokaryotic microorganism, Candida albicans and Trichophyton mentagrophytes as the representative of eukaryotic microorganisms, the inhibition of prokaryotic and eukaryotic microorganisms by silver ion solution under light irradiation was tested and compared Proliferation effect. The experimental results show that silver ions in the solution under irradiation of light have a much greater inhibitory effect on the proliferation of these two types of microorganisms than the solution of silver ions without light. By using electron-spin resonance method, a large amount of hydroxyl radicals (· OH) generated by the photocatalytic reaction of silver ions is detected by using a radical scavenger, that is, reactive oxygen species (· OH) is generated in the solution upon light irradiation. It can be assumed that the solution of reactive oxygen species (· OH) and silver ions in the light irradiation simultaneously attack microbial cells, destroy the cell wall (or cell membrane) and intracellular enzymes, genes, etc., so that the solution shows a strong ability to remove bacteria.