论文部分内容阅读
土壤磷多以有机态形式存在,而植酸磷占一半以上,分解利用植酸态磷是提高土壤磷利用效率、改善植物磷素营养的新途径。利用大豆农杆菌介导子叶节转化与花粉管通道转化技术,将含有根特异启动子pyk10、信号肽S和植酸酶phyA的嵌和基因(KSA)转入冀豆12、冀豆16、五星1号和吉林35中。经PCR检测,共获得T0阳性植株114个,T1阳性植株101个,T2阳性材料28个。通过将T4代转基因株系和野生型对照在仅含植酸磷的营养液中进行培养发现,转基因植株在植酸磷条件下生长状况优于对照,并且3个转基因株系根系分泌型植酸酶活性分别比野生型提高5%、13%和24%。
Phosphorus mostly exists in organic form, while phytic acid phosphorus accounts for more than half. Decomposition and utilization of phytate phosphorus is a new way to improve soil phosphorus utilization efficiency and improve plant phosphorus nutrition. Using Agrobacterium tumefaciens-mediated cotyledonary node transformation and pollen tube channel transformation technology, the inbred gene (KSA) containing the root-specific promoter pyk10, signal peptide S and phytase phyA was transferred into Jidou 12, Jidou 16, 1 and Jilin 35. A total of 114 T0-positive plants, 101 T1-positive plants and 28 T2-positive cells were detected by PCR. By cultivating the T4 generation transgenic lines and the wild-type control in the nutrient solution containing only phytic acid and phosphorus, the transgenic plants were better than the control under phytate phosphorus condition, and the three transgenic lines were root-secreted phytic acid Enzyme activity increased by 5%, 13% and 24% respectively compared with wild type.